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KMID : 1024420090130040314
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Food Engineering Progress
2009 Volume.13 No. 4 p.314 ~ p.319
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Purification of Carp Serum Metallothionein Induced in Carp with Cadmium and Production of Polyclonal Antibody
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Ryu Hyung-Seok
Kang Ho-Joon
Kim Seul-Ki
Kim Nam-Soo
Kim Woo-Yeon
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Abstract
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Metallothioneins (MTs) are low molecular weight, cysteine-rich, and heavy metal binding proteins, which could be induced with heavy metals such as Cd, Hg, Zn and Cu in liver, kidney, and in cultured cells. By using ion exchange chromatography on DE-52, MT was purified from the serum of carp induced with cadmium in order to produce antibody against MT. Polyclonal antibody produced against purified carp MT reacted well with MT in the serum of carp induced with cadmium, whereas control serum did not. This may indicate that the polyclonal antibody against the carp MT could be used for the preparation of biosensors to detect MT in fishes like carp.
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KEYWORD
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carp metallothionein, purification, antibody, reactivity
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